Document Type
Article
Publication Date
2014
Publication Title
Biochemical and Biophysical Research Communications
Volume
443
Issue
2
Pages
568-573
DOI
10.1016/j.bbrc.2013.12.004
Abstract
Multiple studies have shown that bipolar (BP) electric pulses in the microsecond range are more effective at permeabilizing cells while maintaining similar cell survival rates as compared to monopolar (MP) pulse equivalents. In this paper, we investigated whether the same advantage existed for BP nanosecond-pulsed electric fields (nsPEF) as compared to MP nsPEF. To study permeabilization effectiveness, MP or BP pulses were delivered to single Chinese hamster ovary (CHO) cells and the response of three dyes, Calcium Green-1, propidium iodide (PI), and FM1-43, was measured by confocal microscopy. Results show that BP pulses were less effective at increasing intracellular calcium concentration or PI uptake and cause less membrane reorganization (FM1-43) than MP pulses. Twenty-four hour survival was measured in three cell lines (Jurkat, U937, CHO) and over ten times more BP pulses were required to induce death as compared to MP pulses of similar magnitude and duration. Flow cytometry analysis of CHO cells after exposure (at 15 min) revealed that to achieve positive FITC-Annexin V and PI expression, ten times more BP pulses were required than MP pulses. Overall, unlike longer pulse exposures, BP nsPEF exposures proved far less effective at both membrane permeabilization and cell killing than MP nsPEF.
Original Publication Citation
Ibey, B. L., Ullery, J. C., Pakhomova, O. N., Roth, C. C., Semenov, I., Beier, H. T., . . . Pakhomov, A. G. (2014). Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses. Biochemical and Biophysical Research Communications, 443(2), 568-573. doi:10.1016/j.bbrc.2013.12.004
Repository Citation
Ibey, Bennett L.; Pakhomova, Olga N.; Roth, Caleb C.; Xiao, Shu; Schoenbach, Karl; and Pakhomov, Andrei G., "Bipolar Nanosecond Electric Pulses are Less Efficient at Electropermeabilization and Killing Cells than Monopolar Pulses" (2014). Bioelectrics Publications. 171.
https://digitalcommons.odu.edu/bioelectrics_pubs/171
ORCID
0000-0003-4950-4130 (Pakhomova)
Comments
NOTE: This is the author's post-print version of a work that was published in Biochemical and Biophysical Research Communications. The final version was published as:
Ibey, B. L., Ullery, J. C., Pakhomova, O. N., Roth, C. C., Semenov, I., Beier, H. T., . . . Pakhomov, A. G. (2014). Bipolar nanosecond electric pulses are less efficient at electropermeabilization and killing cells than monopolar pulses. Biochemical and Biophysical Research Communications, 443(2), 568-573. doi:10.1016/j.bbrc.2013.12.004
Available at: http://dx.doi.org/10.1016/j.bbrc.2013.12.004