Fluorescence In Situ Hybridization on Sperm Using Alkaline Denaturation

Authors

L. J. Aridgides
M. Stacey, Old Dominion UniversityFollow
L. Brihn
D. Scott
C. Osgood, Old Dominion UniversityFollow

Document Type

Article

Publication Date

2002

Publication Title

Biotechniques

Volume

33

Issue

2

Pages

266-267

Abstract

Fluorescence in situ hybridization (FISH) permits the detection of unique DNA sequences of target DNA within single cells. FISH allows the identifica­tion of DNA sequences within individ­ual cells and readily identifies genetic changes within a mixed population of cells. However, problems arise in main­taining nuclear morphology, particular­ly following harsh denaturation proce­dures where cells are heated to 80°C, causing the nuclear boundary to be­come blurred or the cell to completely burst. In an attempt to preserve cellular morphology and high hybridization ef­ficiency, particularly in fresh tissue samples, we evaluated a FISH protocol using alkaline denaturation in place of heat.

Original Publication Citation

Aridgides, L. J., Stacey, M., Brihn, L., Scott, D., & Osgood, C. (2002). Fluorescence in situ hybridization on sperm using alkaline denaturation. Biotechniques, 33(2), 266-267.

Repository Citation

Aridgides, L. J.; Stacey, M.; Brihn, L.; Scott, D.; and Osgood, C., "Fluorescence In Situ Hybridization on Sperm Using Alkaline Denaturation" (2002). Bioelectrics Publications. 211.
https://digitalcommons.odu.edu/bioelectrics_pubs/211

ORCID

0000-0002-3807-6233 (Stacey)