Document Type

Article

Publication Date

7-2020

Publication Title

Journal of Cellular and Molecular Medicine

Volume

24

Issue

15

Pages

8772–8778

DOI

10.1111/jcmm.15511

Abstract

In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood-brain barrier (BBB) breakdown. After intravenous or intra-arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to promote neurovascular repair. Autologous EPCs transfected to express specific therapeutic proteins offer an innovative therapeutic option. Here, we demonstrate that EPC transfection by electroporation with plasmids encoding the reporter protein GFP or an anti-beta-amyloid antibody fragment (Fab) leads to secretion of each protein. We also demonstrate the secreted anti-beta-amyloid Fab protein functions in beta-amyloid aggregate solubilization.

Comments

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

© 2020 ALSaTECH. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

Early access are articles that have been peer-reviewed and accepted for publication.The article content has been finalized, but it has not been assigned to an issue yet.

Original Publication Citation

Heller, L., Thinard, R., Chevalier, M., Arpag, S., Jing, Y., Greferath, R., ... & Nicolau, C. Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells. Journal of Cellular and Molecular Medicine, 24(15), 8772–8778. https://doi.org/10.1111/jcmm.15511

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