Document Type

Article

Publication Date

2020

Publication Title

Microorganisms

Volume

8

Issue

7

Pages

1-14

DOI

10.3390/microorganisms8071091

Abstract

Cold atmospheric plasma (CAP) has been widely used in biomedicine during the last two decades. While direct plasma treatment has been reported to promote wound healing, its application can be uneven and inconvenient. In this study, we first activated water with a portable dielectric barrier discharge plasma device and evaluated the inactivation effect of plasma-activated water (PAW) on several kinds of bacteria that commonly infect wounds. The results show that PAW can effectively inactivate these bacteria. Then, we activated tap water and examined the efficacy of PAW on wound healing in a mouse model of full-thickness skin wounds. We found that wound healing in mice treated with PAW was significantly faster compared with the control group. Histological analysis of the skin tissue of mice wounds showed a significant reduction in the number of inflammatory cells in the PAW treatment group. To identify the possible mechanism by which PAW promotes wound healing, we analyzed changes in the profiles of wound bacteria after PAW treatment. The results show that PAW can significantly reduce the abundance of wound bacteria in the treatment group. The results of biochemical blood tests and histological analysis of major internal organs in the mice show that PAW had no obvious side effects. Taken together, these results indicate that PAW may be a new and effective method for promoting wound healing without side effects.

Comments

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Original Publication Citation

Xu, D., Wang, S., Li, B., Qi, M., Feng, R., Li, Q., Zhang, H., Chen, H., & Kong, M. G. (2020). Effects of plasma-activated water on skin wound healing in mice. Microorganisms, 8(7), 1-14, Article 1091. https://doi.org/10.3390/microorganisms8071091

ORCID

0000-0001-8142-4726 (Kong)

Included in

Biomedical Commons

Share

COinS