Document Type
Article
Publication Date
2021
Publication Title
Bioelectrochemistry
Volume
142
Pages
107892 (1-7)
DOI
10.1016/j.bioelechem.2021.107892
Abstract
Damage from myocardial infarction (MI) and subsequent heart failure are serious public health concerns. Current clinical treatments and therapies to treat MI damage largely do not address the regeneration of cardiomyocytes. In a previous study, we established that it is possible to promote regeneration of cardiac muscle with vascular endothelial growth factor B gene delivery directly to the ischemic myocardium. In the current study we aim to optimize cardioporation parameters to increase expression efficiency by varying electrode configuration, applied voltage, pulse length, and plasmid vector size. By using a surface monopolar electrode, optimized pulsing conditions and reducing vector size, we were able to prevent ventricular fibrillation, increase survival, reduce tissue damage, and significantly increase gene expression levels.
Original Publication Citation
Boye, C., Arpag, S., Burcus, N., Lundberg, C., DeClemente, S., Heller, R., Francis, M., & Bulysheva, A. (2021). Cardioporation enhances myocardial gene expression in rat heart. Bioelectrochemistry, 142, 1-7, Article 107892. https://doi.org/10.1016/j.bioelechem.2021.107892
Repository Citation
Boye, Carly; Arpag, Sezgi; Burcus, Nina; Lundberg, Cathryn; DeClemente, Scott; Heller, Richard; Francis, Michael; and Bulysheva, Anna, "Cardioporation Enhances Myocardial Gene Expression in Rat Heart" (2021). Bioelectrics Publications. 309.
https://digitalcommons.odu.edu/bioelectrics_pubs/309
ORCID
0000-0002-7287-9371 (Bulysheva)
Comments
© 2021 The Authors.
This is an open access article under the Creative Commons Attribution 4.0 International License.