Document Type
Article
Publication Date
2016
Publication Title
Scientific Reports
Volume
6
Pages
1-14
DOI
10.1038/srep27872
Abstract
This study reports the potential of cold atmospheric plasma (CAP) as a versatile tool for delivering oligonucleotides into mammalian cells. Compared to lipofection and electroporation methods, plasma transfection showed a better uptake efficiency and less cell death in the transfection of oligonucleotides. We demonstrated that the level of extracellular aqueous reactive oxygen species (ROS) produced by gas plasma is correlated with the uptake efficiency and that this is achieved through an increase of intracellular ROS levels and the resulting increase in cell membrane permeability. This finding was supported by the use of ROS scavengers, which reduced CAP-based uptake efficiency. In addition, we found that cold atmospheric plasma could transfer oligonucleotides such as siRNA and miRNA into cells even in 3D cultures, thus suggesting the potential for unique applications of CAP beyond those provided by standard transfection techniques. Together, our results suggest that cold plasma might provide an efficient technique for the delivery of siRNA and miRNA in 2D and 3D culture models.
Original Publication Citation
Xu, D. H., Wang, B. Q., Xu, Y. J., Chen, Z. Y., Cui, Q. J., Yang, Y. J., . . . Kong, M. G. (2016). Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures. Scientific Reports, 6, 1-14. doi: 10.1038/srep27872
Repository Citation
Xu, Dehui; Wang, Biqing; Xu, Yujing; Chen, Zeyu; Cui, Qinjie; Yang, Yanjie; Chen, Hailan; and Kong, Michael G., "Intracellular ROS Mediates Gas Plasma-Facilitated Cellular Transfection in 2D and 3D Cultures" (2016). Bioelectrics Publications. 99.
https://digitalcommons.odu.edu/bioelectrics_pubs/99
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Bioelectrical and Neuroengineering Commons, Biomedical Commons, Medical Sciences Commons