Date of Award
Summer 1999
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
Program/Concentration
Biology
Committee Director
Wayne L. Hynes
Committee Member
Keith A. Carson
Committee Member
Christopher Osgood
Call Number for Print
Special Collections LD4331.B46 J356
Abstract
The inhibitory activity exhibited by Staphylococcus hominis strain αM toward Mycobacterium species was examined. These studies included determining the conditions that maximized inhibitory agent production, analyzing characteristics of the agent, determining the time of agent production, ascertaining plasmid possession and the role of plasmids in the production of this inhibitory agent, and ascertaining the antimicrobial activity of other strains of S. hominis. On solid and in liquid media, S. hominis strain αM exhibits a unique inhibitory activity toward Mycobacterium species that is not demonstrated by Staphylococcus epidermidis or other S. hominis strains. Maximum agent production and recovery was accomplished when S. Hominis αM, inoculated in either Tryptic soy broth or Todd-Hewitt broth, was incubated for at least 18 hours at 37°C under aerobic (shaking) conditions. The agent was subjected to one-step 60% ammonium sulfate precipitation and polyethylene glycol concentration. The molecular weight of the agent was estimated by membrane filtration to be between 12000 and 30000 MW and was characterized as containing a heat-stable, acid-stable, and base-labile biologically active protein moiety. This agent displays a broad spectrum of inhibitory activity toward gram-positive bacteria but showed little effect on gram-negative organisms. Growth of S. hominis strain αM consists of a lag phase, with production of the agent occurring during logarithmic phase. S. hominis strain αM potentially possesses up to seven plasmids when examined electrophoretically and curing with various agents did not cease the anti-Mycobacterial activity.
Rights
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DOI
10.25777/1mgp-am98
Recommended Citation
Jacobsen, Sandra M..
"An Extracellular Protein Produced by Staphylococcus Hominis Strain αM with Antimicrobial Activity Against Mycobacterium SPP"
(1999). Master of Science (MS), Thesis, Biological Sciences, Old Dominion University, DOI: 10.25777/1mgp-am98
https://digitalcommons.odu.edu/biology_etds/209