Date of Award

Spring 1999

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Barbara Hargrave

Committee Member

Edward Poziomek

Committee Member

Christopher J. Osgood

Call Number for Print

Special Collections LD4331.B46 C416

Abstract

The purpose of this study is to determine the effect of acute administration of heroin and ecgonine methyl ester (EME) on renin and atrial natriuretic peptide (ANP) secretion in time dated New Zealand White rabbits. Thirteen rabbits were studied. Arterial blood pressure and pCOz, increased significantly from a control value of 80 ± 8 mmHg and 30 ± 2 mmHg respectively to 93 ± 18 mmHg and 31 ± 1 mmHg. pO2 decreased from 99 ± 8 mmHg to 90 ± 11 mmHg 10 minutes after heroin administration. EME caused arterial blood pressure to significantly increase from a control value of 68.5 ± 4.4 mmHg to 75.7 ± 4.1 mmHg. Ion mobility spectrometry (IMS) was used to detect EME, heroin and/or its metabolites in maternal and fetal tissues. Neither heroin nor any of its metabolites were detected in rabbit tissue after the acute administration of 200 µg of heroin, but EME(2 mg/kg) was detected in animals after acute administration. There were no statistically significant changes in PRA, HCT, or protein. There was a significant increase in ANP secretion from a control value of 34 ± 9.8 pg/ml to 41 ± 10.6 pg/ml 10 minutes after heroin administration. Acute EME administration caused no significant changes in pCO2, pO2, hematocrit, or protein content. There was a significant increase in plasma renin activity 5 minutes after administration from a control value of 7.94 ± 9 ng/ml/hr to 18.7 ± 3.1 ng/ml/hr. These data suggest that the acute exposure to Heroin does not alter the secretion of renin, but does alter ANP secretion in pregnant rabbits. The acute exposure to EME alters plasma renin activity. Furthermore, it has been found the use of IMS for heroin detection in maternal and fetal tissue is not feasible because of heroin IMS signal retardation in the presence of animal tissue, but is feasible for EME detection.

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DOI

10.25777/2a0x-gd59

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