Document Type

Article

Publication Date

2015

DOI

10.4049/jimmunol.1400887

Publication Title

Journal of Immunology

Volume

194

Issue

11

Pages

5261-5271

Abstract

Type 1 diabetes is one of the most extensively studied autoimmune diseases, but the cellular and molecular mechanisms leading to T cell–mediated destruction of insulin-producing β cells are still not well understood. In this study, we show that regulatory T cells (Tregs) in NOD mice undergo age-dependent loss of suppressor functions exacerbated by the decreased ability of activated effector T cells to upregulate Foxp3 and generate Tregs in the peripheral organs. This age-dependent loss is associated with reduced intercellular communication mediated by gap junctions, which is caused by impaired upregulation and decreased expression of connexin 43. Regulatory functions can be corrected, even in T cells isolated from aged, diabetic mice, by a synergistic activity of retinoic acid, TGF-β, and IL-2, which enhance connexin 43 and Foxp3 expression in Tregs and restore the ability of conventional CD4+ T cells to upregulate Foxp3 and generate peripherally derived Tregs. Moreover, we demonstrate that suppression mediated by Tregs from diabetic mice is enhanced by a novel reagent, which facilitates gap junction aggregation. In summary, our report identifies gap junction–mediated intercellular communication as an important component of the Treg suppression mechanism compromised in NOD mice and suggests how Treg mediated immune regulation can be improved.

Rights

Web of Science: "Free full-text from publisher."

Original Publication Citation

Kuczma, M., Wang, C. Y., Ignatowicz, L., Gourdie, R., & Kraj, P. (2015). Altered connexin 43 expression underlies age-dependent decrease of regulatory T cell suppressor function in nonobese diabetic mice. Journal of Immunology, 194(11), 5261-5271. doi:10.4049/jimmunol.1400887

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