Date of Award
Summer 1998
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry & Biochemistry
Program/Concentration
Chemistry
Committee Director
Frank E. Scully, Jr.
Committee Member
Roy L. Williams
Committee Member
John R. Donat
Call Number for Print
Special Collections LD4331.C45 C74
Abstract
The N-terminal amino acid residues on polypeptides and proteins were determined in wastewaters prior to chlorination. The terminal amino groups'ere first derivatized with the well known derivatizing agent dansyl chloride, and then the resulting dansyl amino acid hydrolyzed from the peptide chain by a propionic acid/ hydrochloric acid mixture. The resulting dansyl amino acids were then separated and detected using reversed phase high performance liquid chromatography with fluorescence detection. The majority of N-terminal residues detected were the more polar amino acids. Concentrations ranged from 1 x 10-10 to 3 x 10-7 moles/liter. It is suggested that the shorter chain length polypeptides present in wastewaters have polar N-terminal amino acid residues. The nonpolar N-terminal amino acid residues do not make a significant contribution to the chlorine demand of organic amino nitrogen in wastewaters.
DOI
10.25777/6kjv-yj52
Recommended Citation
Creecy, Edward L..
"Determination of the N-Terminal Amino Acid Residues on Polypeptides in Secondary Wastewaters"
(1998). Master of Science (MS), Thesis, Chemistry & Biochemistry, Old Dominion University, DOI: 10.25777/6kjv-yj52
https://digitalcommons.odu.edu/chemistry_etds/100
Included in
Amino Acids, Peptides, and Proteins Commons, Organic Chemistry Commons, Water Resource Management Commons