Date of Award
Spring 1981
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry & Biochemistry
Program/Concentration
Chemistry
Committee Director
Roy L. Williams
Committee Member
Frank E. Scully, Jr.
Committee Member
Billy T. Upchurch
Committee Member
Perry M. Duncan
Call Number for Print
Special Collections LD4331.C45 K44
Abstract
The objectives of this research were to develop a sensitive method for separation and quantitation of biogenic amines and to apply that procedure by monitoring neurochemical changes resulting from stimuli to the test system.
Primary aromatic amines were derivatized with o-phthalaldehyde-mercaptoethanol prior to chromatography, Separation of the fluorescent adduct was achieved using a μBondapak/ phenyl reversed-phase column and a two-step gradient of methanol:0.08 M acetic acid, pH J.O. Fluorescence was monitored using a J40 nm excitation energy and an emission filter which transmits radiation greater than 400 nm (50% T at 418 nanometers).
Following optimization of the chromatographic conditions, studies of the extraction of biogenic amines from biological materials were carried out. Results indicated that perchloric acid deproteinization followed by ethyl acetate extraction of the fluorescent derivative produced unidentified substances which interfered with accurate quantitation of the biogenic amines.
Rights
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DOI
10.25777/1724-vv63
Recommended Citation
Keegan, Thais E..
"High Performance Liquid Chromatographic Analysis of Biogenic Amines"
(1981). Master of Science (MS), Thesis, Chemistry & Biochemistry, Old Dominion University, DOI: 10.25777/1724-vv63
https://digitalcommons.odu.edu/chemistry_etds/123