Date of Award

Spring 1981

Document Type


Degree Name

Master of Science (MS)


Chemistry & Biochemistry



Committee Director

Roy L. Williams

Committee Member

Frank E. Scully, Jr.

Committee Member

Billy T. Upchurch

Committee Member

Perry M. Duncan

Call Number for Print

Special Collections LD4331.C45 K44


The objectives of this research were to develop a sensitive method for separation and quantitation of biogenic amines and to apply that procedure by monitoring neurochemical changes resulting from stimuli to the test system.

Primary aromatic amines were derivatized with o-phthalaldehyde-mercaptoethanol prior to chromatography, Separation of the fluorescent adduct was achieved using a μBondapak/ phenyl reversed-phase column and a two-step gradient of methanol:0.08 M acetic acid, pH J.O. Fluorescence was monitored using a J40 nm excitation energy and an emission filter which transmits radiation greater than 400 nm (50% T at 418 nanometers).

Following optimization of the chromatographic conditions, studies of the extraction of biogenic amines from biological materials were carried out. Results indicated that perchloric acid deproteinization followed by ethyl acetate extraction of the fluorescent derivative produced unidentified substances which interfered with accurate quantitation of the biogenic amines.


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