Date of Award

Spring 1980

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

Kenneth G. Brown

Committee Member

Patricia A. Pleban

Committee Member

Mark S. Elliott

Call Number for Print

Special Collections LD4331.C45M65

Abstract

FTIR was used in conjunction with a surface selective technique, known as attenuated total reflectance (ATR), to study the infrared spectra of preimplantation rabbit embryos. The rabbit embryos ranged in aged from 3 to 6 days. Since an embryo is mostly water, deuteration of the embryo was necessary to eliminate the intense water background present in the mid-infrared region. A set of spectral standards were tested, in order to identify the bands seen in embryo spectra. By analyzing and comparing the spectra from 3, 4, 5 and 6 day embryos, differences could be noted. In particular, bands defined as Amide I and II and some bands attributed to lipids showed marked changes. These differences are believed to be indicative of structural changes that take place as the embryo develops.

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DOI

10.25777/wfeg-9594

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