Date of Award

Spring 1980

Document Type


Degree Name

Master of Science (MS)


Chemistry & Biochemistry



Committee Director

Kenneth G. Brown

Committee Member

Patricia A. Pleban

Committee Member

Mark S. Elliott

Call Number for Print

Special Collections LD4331.C45M65


FTIR was used in conjunction with a surface selective technique, known as attenuated total reflectance (ATR), to study the infrared spectra of preimplantation rabbit embryos. The rabbit embryos ranged in aged from 3 to 6 days. Since an embryo is mostly water, deuteration of the embryo was necessary to eliminate the intense water background present in the mid-infrared region. A set of spectral standards were tested, in order to identify the bands seen in embryo spectra. By analyzing and comparing the spectra from 3, 4, 5 and 6 day embryos, differences could be noted. In particular, bands defined as Amide I and II and some bands attributed to lipids showed marked changes. These differences are believed to be indicative of structural changes that take place as the embryo develops.


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