Date of Award

Spring 2008

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

Michael J. Solhaug

Committee Member

Patricia A. Pleban

Committee Member

Terry A. Jacot

Call Number for Print

Special Collections LD4331.C45 M66 2008

Abstract

This purpose of this study was to examine the regulation of endothelial and neuronal nitric oxide synthase expression in the preglomerular vessels of mature and immature porcine kidneys by the increase in intracellular calcium concentration. Angiotensin II can increase intracellular calcium concentrations in two ways; either through the mobilization of intracellular calcium stores or through the influx of calcium from the extracellular fluid through calcium channels. The aim of this study was to determine the effects of a calcium control, a calcium-free control, L- and T-type voltage-gated calcium channel blockers, an internal calcium store release agent, KCI, and EDTA on endothelial and neuronal nitric oxide synthase (eNOS and nNOS respectively) expression in preglomerular vessels. Findings suggest that eNOS expression is regulated by the mobilization of intracellular calcium stores by means of store-operated calcium channels and nNOS expression is regulated through the influx of calcium from the extracellular fluid through L-type voltage-gated calcium channels.

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DOI

10.25777/sj2r-0m61

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