Date of Award

Summer 1990

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

Mark S. Elliot

Committee Member

Patricia A. Pleban

Committee Member

Roy L. Williams

Call Number for Print

Special Collections LD4331.C45W57

Abstract

Transfer RNA (tRNA), is a complex class of small molecules which have been proven to play a major role in protein synthesis. Transfer RNA is composed of 20 families of isoacceptors which may recognize only one particular amino acid. This family of isoacceptors may differ from each other by primary sequence or modified nucleosides. Queuine is a modified nucleoside which is found in the anticodon regions of tRNA isoacceptors for asparagine, aspartic acid, tyrosine and histidine. Queuine has gained the interest of many researchers since queuine deficient tRNA was found in many neoplastic and undifferentiated cell-lines. It is often difficult to isolate those tRNA isoacceptors which have critical modifications such as queuine. One major reason for this is the limitation of the resolution obtainable in

chromatographic isolation procedures. Here we demonstrate a technique to separate out queuine containing isoacceptors which appears to be more useful than those techniques previously utilized. With a revised gradient and buffer system the W-Porex C-4 HPLC column by Phenomenex has proven to give excellent resolution of the spectrum of tRNA molecules from several sources. We have identified some of these tRNA isoacceptors and pinpointed which of these molecules contained queuine. This new technique is useful in examining isoacceptors which contain queuine and may be an important tool to aid in the discovery of the role of this modified nucleoside in neoplastic cells.

Rights

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DOI

10.25777/zr0r-n156

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