Date of Award

Spring 2002

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry & Biochemistry

Committee Director

James H. Yuan

Committee Member

Mark S. Elliot

Committee Member

Roy L. Williams

Call Number for Print

Special Collections LD4331.B48 A97 2002

Abstract

Benzo[α]pyrene is a ubiquitous pollutant produced from the incomplete combustion of organic material such as fossil fuels. It is found in the workplace, urban air, drinking water, and the food supply. Recently, it has been proposed that benzo[α]pyrene may be the causative agent in the formation of lung adenocarcinomas among some Taiwanese women exposed to cooking oil fumes without adequate ventilation. In this study, calf thymus DNA was modified in vitro with benzo[α]pyrene-diol epoxide (BPDE) to a level consistent with that found in biological samples. This DNA of low modification was used as an immunogen in the production of a monoclonal antibody specific for the detection of benzo[α]pyrene-DNA adducts and used in the development of a sensitive colorimetric enzyme-linked immunosorbant assay (ELISA). Following it's development, the newly developed assay will be used independently in a subsequent epidemiological study to explore the correlation between exposure to benzopyrene generated from hot cooking oils, adduct formation, and the generation of relevant carcinomas.

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DOI

10.25777/ezpx-9g52

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