Date of Award

Fall 1989

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Clinical Chemistry

Committee Director

Patricia A. Pleban

Committee Member

James H. Yuan

Committee Member

Anibal Acosta

Committee Member

Sergio Oehninger

Call Number for Print

Special Collections LD4331.C45T34

Abstract

High performance liquid chromatography (HPLC)methodologies for the determination of tamoxifen and its metabolites 4-hydroxytamoxifen and n-desmethyltamoxifen, and using cis-tamoxifen as an internal standard have been developed.

Clinical analyses of tamoxifen and its metabolites in human serum and semen, were performed as follows: The analytes were extracted from serum and semen with hexane, the hexane evaporated, the dried extract then reconstituted with methanol, followed by reverse phase high performance liquid chromatography.

Between-run coefficients of variation (CV'S) for tamoxifen and n-desmethyltamoxifen for serum sample analyses, (n = 15) were, 4% and 13% at mean (SD) concentration of 140.2 (6.2) and 71.8 (9.8) ug/1, respectively.

Recovery studies of 4-hydroxytamoxifen, n-desmethyltamoxifen and tamoxifen from spiked, pooled serum and semen gave mean values 25%, 55%, 96% for serum, and for semen 46%, 66% and 95%, respectively.

Human serum 4-hydroxytamoxifen, n-desmethyltamoxifen, and tamoxifen, concentrations were determined in 23 specimens obtained from patients undergoing studies for infertility. The possible relationships between serum and semen concentrations and FSH, LH, testosterone, and sperm quality were investigated.

When the Spearman correlation coefficients were determined, serum levels of n-desmethyltamoxifen correlated significantly and positively with serum FSH, LH and serum testosterone levels. Semen n-desmethyltamoxifen levels correlated significantly and positively with semen motility and negatively with semen sperm concentration. Concentration of serum tamoxifen correlated positively and significantly with serum n-desmethyltamoxifen levels.

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DOI

10.25777/yj6c-xp43

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