Date of Award
Fall 1991
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry & Biochemistry
Program/Concentration
Chemistry
Committee Director
Patricia A. Pleban
Committee Member
Mark S. Elliot
Committee Member
Myuang H. Kim
Committee Member
Roy L. Williams
Call Number for Print
Special Collections LD4331.C45C48
Abstract
The objective of this research was to develop a method for the separation of different types of collagen using a large pore size (100 nm) reversed-phase C-8 column. Three different types of collagen (type I, III, and V) were sufficiently separated on the column using a mobile phase containing water-acetonitrile-trifluoroacetic acid. Collagens found in each HPLC peak were identified using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to assay the column effluents.
Trifluoroacetic acid (TFA) was used as an ion pair reagent. This compound improved the chromatographic profile for these proteins on a reversed-phase column. It was observed that with higher TFA concentration, the collagens eluted more quickly.
Type I and type III collagen were also isolated from placental tissue both by DEAE-anion exchange cellulose column and by salt precipitation. The isolated proteins were applied to the reversed phase HPLC column and were detected using SDSPAGE.
Rights
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
DOI
10.25777/vn89-jq66
Recommended Citation
Chung, Yongjoo.
"High Performance Liquid Chromatographic Separation of Different Types of Collagen"
(1991). Master of Science (MS), Thesis, Chemistry & Biochemistry, Old Dominion University, DOI: 10.25777/vn89-jq66
https://digitalcommons.odu.edu/chemistry_etds/90