Date of Award
Fall 1987
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry & Biochemistry
Program/Concentration
Chemistry
Committee Director
Thomas O. Sitz
Committee Member
Kenneth G. Brown
Committee Member
Robert L. Ake
Call Number for Print
Special Collections LD4331.C45D38
Abstract
Duplex formation between oligo(C:G) n where n=3 to 4 was shown not to occur under conditions favorable for duplex formation between poly G and poly C. Instead, a stable guano sine self-structure was found to form which a Tm of 50°C for (Gp)3 and 80°C for (Gp)4 at strand concentrations of 10-5M in 1M NaCl. Neither a duplex nor a self-structure formed in the absence of salt.
Oligomers of guanosine and cytosine were obtained by basic hydrolysis and separated according to chain length using DEAE Sephadex column chromatography. Separation of cytosine oligomers with chain lengths up to eight bases and oligomers of guanosine up to four bases was achieved. A brown oxidation product formed during the hydrolysis of poly G but did not occur when the reaction solutions were purged with He prior to hydrolysis.
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DOI
10.25777/cfes-5e61
Recommended Citation
Davis, Steven R..
"Investigation of Complex Formation by Oligomers of Cytosine and Guanosine"
(1987). Master of Science (MS), Thesis, Chemistry & Biochemistry, Old Dominion University, DOI: 10.25777/cfes-5e61
https://digitalcommons.odu.edu/chemistry_etds/99
Included in
Biochemistry Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons, Organic Chemistry Commons, Polymer Chemistry Commons