Date of Award

Fall 1987

Document Type


Degree Name

Master of Science (MS)


Chemistry & Biochemistry



Committee Director

Thomas O. Sitz

Committee Member

Kenneth G. Brown

Committee Member

Robert L. Ake

Call Number for Print

Special Collections LD4331.C45D38


Duplex formation between oligo(C:G) n where n=3 to 4 was shown not to occur under conditions favorable for duplex formation between poly G and poly C. Instead, a stable guano sine self-structure was found to form which a Tm of 50°C for (Gp)3 and 80°C for (Gp)4 at strand concentrations of 10-5M in 1M NaCl. Neither a duplex nor a self-structure formed in the absence of salt.

Oligomers of guanosine and cytosine were obtained by basic hydrolysis and separated according to chain length using DEAE Sephadex column chromatography. Separation of cytosine oligomers with chain lengths up to eight bases and oligomers of guanosine up to four bases was achieved. A brown oxidation product formed during the hydrolysis of poly G but did not occur when the reaction solutions were purged with He prior to hydrolysis.


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