Document Type

Article

Publication Date

2008

DOI

10.4319/lom.2008.6.246

Publication Title

Limnology and Oceanography: Methods

Volume

6

Pages

246-253

Abstract

The analysis of dissolved organic matter ( DOM) by Fourier transform ion cyclotron resonance mass spectrometry ( FTICR- MS) has gained wide interest recently, driven primarily by its ultrahigh resolving power and mass accuracy. Accurate calibration of mass spectra is a key step to successfully decipher the DOM components. We propose a simple and accurate method to internally calibrate the peaks in the complex spectra without the need to add a calibrant. Mass spectra of DOM samples from the Dismal Swamp, Virginia, and the lower Chesapeake Bay display the presence of naturally occurring fatty acids which can be readily recognized and calibrated with accuracies < 0.1 ppm. Once calibrated with fatty acids, approximately 80% of all peaks in the DOM mass spectra can be assigned unique molecular formulas with accuracies < 0.4 ppm. Although the formula errors for the assigned molecular formulas do increase with increasing m/z, the dynamic range of the fatty acids used as calibrants is sufficient because high m/z values (> 600) have formulas with an average error of < 0.6 ppm. Because fatty acids are ubiquitous components of most DOM, this approach is applicable to a large variety of DOM samples. © 2008, by the American Society of Limnology and Oceanography, Inc.

Comments

Web of Science: "Free full-text from publisher -- gold open access."

Original Publication Citation

Sleighter, R. L., McKee, G. A., Liu, Z., & Hatcher, P. G. (2008). Naturally present fatty acids as internal calibrants for fourier transform mass spectra of dissolved organic matter. Limnology and Oceanography: Methods, 6, 246-253. doi:10.4319/lom.2008.6.246

ORCID

0000-0002-1606-1305 (Hatcher)

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