Document Type

Article

Publication Date

2023

DOI

10.1039/D3CB00035D

Publication Title

RSC Chemical Biology

Volume

4

Pages

573-586

Abstract

Metalation of the N-terminal Amino Terminal Cu(II)- and Ni(II)-binding (ATCUN) motif may enhance the antimicrobial properties of piscidins. Molecular dynamics simulations of free and nickelated piscidins 1 and 3 (P1 and P3) were performed in 3 : 1 POPC/POPG and 2.6 : 1 : 0.4 POPC/POPG/aldo-PC bilayers (POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine: POPG, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol; aldo-PC, 1-palmitoyl-2-(9′-oxo-nonanoyl)-sn-glycero-3-phosphocholine) bilayer models. Nickel(II) binding decreases the conformation dynamics of the ATCUN motif and lowers the charge of the N-terminus to allow it to embed deeper in the bilayer without significantly changing the overall depth due to interactions of the charged half-helix of the peptide with the headgroups. Phe1⋯Ni2+ cation–π and Phe2–Phe1 CH–π interactions contribute to a small fraction of structures within the nickelated P1 simulations and may partially protect a bound metal from metal-centered chemical activity. The substitution of Phe2 for Ile2 in P3 sterically blocks conformations with cation–π interactions offering less protection to the metal. This difference between metalated P1 and P3 may indicate a mechanism by which peptide sequence can influence antimicrobial properties. Any loss of bilayer integrity due to chain reversal of the oxidized phospholipid chains of aldo-PC may be enhanced in the presence of metalated piscidins.

Rights

© 2023 The Authors.

This article is licensed under a Creative Commons Attribution 3.0 Unported (CC BY 3.0) Licence.

Original Publication Citation

Dreab, A., & Bayse, C. A. (2023). The effect of metalation on antimicrobial piscidins imbedded in normal and oxidized lipid bilayers. RSC Chemical Biology, 4, 573-586. https://doi.org/10.1039/d3cb00035d

ORCID

0000-0002-7836-247X (Dreab), 0000-0002-3490-576X (Bayse)

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