Document Type
Article
Publication Date
2020
DOI
10.1128/mmbr.00062-19
Publication Title
Microbiology and Molecular Biology Reviews
Volume
84
Issue
2
Pages
e00062-19 (1-25)
Abstract
Although enteroviruses are associated with a wide variety of diseases and conditions, their mode of replication is well conserved. Their genome is carried as a single, positive-sense RNA strand. At the 5′ end of the strand is an approximately 90-nucleotide self-complementary region called the 5′ cloverleaf, or the oriL. This noncoding region serves as a platform upon which host and virus proteins, including the 3B, 3C, and 3D virus proteins, assemble in order to initiate replication of a negative-sense RNA strand. The negative strand in turn serves as a template for synthesis of multiple positive-sense RNA strands. Building on structural studies of individual RNA stem-loops, the structure of the intact 5′ cloverleaf from rhinovirus has recently been determined via nuclear magnetic resonance/small-angle X-ray scattering (NMR/SAXS)-based methods, while structures have also been determined for enterovirus 3A, 3B, 3C, and 3D proteins. Analysis of these structures, together with structural and modeling studies of interactions between host and virus proteins and RNA, has begun to provide insight into the enterovirus replication mechanism and the potential to inhibit replication by blocking these interactions.
Rights
© 2020 American Society for Microbiology.
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Original Publication Citation
Pascal, S. M., Garimella, R., Warden, M. S., & Ponniah, K. (2020). Structural biology of the enterovirus replication-linked 5'-cloverleaf RNA and associated virus proteins. Microbiology and Molecular Biology Reviews, 84(2), 1-25, Article e00062-19. https://doi.org/10.1128/mmbr.00062-19
Repository Citation
Pascal, Steven M.; Garimella, Ravindranath; Warden, Meghan S.; and Ponniah, Komala, "Structural Biology of the Enterovirus Replication-Linked 5'-Cloverleaf RNA and associated Virus Proteins" (2020). Chemistry & Biochemistry Faculty Publications. 272.
https://digitalcommons.odu.edu/chemistry_fac_pubs/272
Included in
Amino Acids, Peptides, and Proteins Commons, Genetics and Genomics Commons, Virus Diseases Commons, Viruses Commons