Enhancement of Antitumor Effects of Berberine Chloride With a Copper(II) Complex Against Human Triple Negative Breast Cancer: In Vitro Studies

Authors

Duaa R. Alajroush, Old Dominion UniversityFollow
Brittney F. Anderson, University of the Virgin Islands
Janae A. Bruce, University of the Virgin Islands
Christian I. Lartey, Old Dominion UniversityFollow
Dazonte A. Mathurin, University of the Virgin Islands
Sean T. Washington, Old Dominion UniversityFollow
Tanaya S. Washington, Old Dominion UniversityFollow
Sidy Diawara, Old Dominion UniversityFollow
Sakariyau A. Waheed, Old Dominion UniversityFollow
Kaylin L. Thomas, Old Dominion UniversityFollow
Stephen J. Beebe, Old Dominion UniversityFollow
Alvin A. Holder, Old Dominion UniversityFollow

Document Type

Article

Publication Date

2024

DOI

10.1016/j.rechem.2024.101882

Publication Title

Results in Chemistry

Volume

12

Pages

101882

Abstract

In this study, the copper(II) complex [Cu(chromoneTSC)Cl₂]•0.5H₂O•0.0625C₂H₅OH (where chromoneTSC = (E)-N-Ethyl-2-((4-oxo-4H-chromen-3-yl)methylene)-hydrazinecarbothioamide) was synthesized and characterized; then used to carry out in vitro studies in combination with berberine chloride (BBC). The ligand and complex were characterized by elemental analysis, FTIR and NMR (¹H and ¹³C) spectroscopy, and conductivity measurements. The cytotoxic effect was analyzed by using the CCK-8 viability assay in cancer MDA-MB-231 VIM RFP and non-cancer MCF-10A cell lines. The IC₅₀ values for the complex and BBC were 21.2 ± 1.6 and 48.3 ± 2.4 μM, respectively at 24 h incubation, while the IC₅₀ value of the combination treatment was 9.3 ± 1.5 in cancer cells. The co-treatment group significantly increased the number of cells in G2 phase, indicating the growth arrest of cancer cells. Moreover, the combination group showed induction of both intrinsic and extrinsic apoptotic pathways. There was also a study on the effect of the combination treatment on receptor-interacting serine/threonine-protein kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL) as biomarkers of necroptosis. The results showed activation of necroptosis after treatment with the combination of the copper complex and BBC via the activation of RIPK3–MLKL pathway.

Rights

© 2024 The Authors.

This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.

Data Availability

Article states: "Data will be made available on request."

Original Publication Citation

Alajroush, D. R., Anderson, B. F., Bruce, J. A., Lartey, C. I., Mathurin, D. A., Washington, S. T., Washington, T. S., Diawara, S., Waheed, S. A., Thomas, K. L., Beebe, S. J., & Holder, A. A. (2024). Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies. Results in Chemistry, 12, 1-15, Article 101882. https://doi.org/10.1016/j.rechem.2024.101882

ORCID

0009-0004-9697-0155 (Diawara), 0000-0002-4546-0153 (Waheed), 0000-0002-6075-9452 (Beebe), 0000-0001-9618-5297 (Holder)

Repository Citation

Alajroush, Duaa R.; Anderson, Brittney F.; Bruce, Janae A.; Lartey, Christian I.; Mathurin, Dazonte A.; Washington, Sean T.; Washington, Tanaya S.; Diawara, Sidy; Waheed, Sakariyau A.; Thomas, Kaylin L.; Beebe, Stephen J.; and Holder, Alvin A., "Enhancement of Antitumor Effects of Berberine Chloride With a Copper(II) Complex Against Human Triple Negative Breast Cancer: In Vitro Studies" (2024). Chemistry & Biochemistry Faculty Publications. 324.
https://digitalcommons.odu.edu/chemistry_fac_pubs/324