Document Type
Article
Publication Date
2025
DOI
10.1128/jb.00129-25
Publication Title
Journal of Bacteriology
Volume
207
Issue
8
Pages
e0012925
Abstract
Bacterial two-hybrid (B2H) systems offer a versatile platform for detecting protein-protein interactions (PPIs) in vivo. Originally developed to study bacterial transcriptional regulators, these systems have evolved to support a wide range of applications, including interaction mapping, domain analysis, and high-throughput screening. This review surveys the development and expansion of B2H systems across diverse biological contexts. We highlight technical considerations, platform-specific innovations, and recent adaptations that extend the utility of B2H systems in both prokaryotic and eukaryotic protein interaction research environments. By comparing different B2H variants and discussing their integration with complementary approaches, we provide a framework for leveraging these systems in modern PPI discovery.
Rights
© 2025 Richardson and Pascal.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Original Publication Citation
Richardson, R. M., & Pascal, S. M. (2025). Bacterial two-hybrid systems evolved: Innovations for protein-protein interaction research. Journal of Bacteriology, 207(8), Article e0012925. https://doi.org/10.1128/jb.00129-25
ORCID
0009-0000-7213-9552 (Richardson), 0000-0002-9492-6167 (Pascal)
Repository Citation
Richardson, Rebecca M. and Pascal, Steven M., "Bacterial Two-Hybrid Systems Evolved: Innovations for Protein-Protein Interaction Research" (2025). Chemistry & Biochemistry Faculty Publications. 360.
https://digitalcommons.odu.edu/chemistry_fac_pubs/360
NGB2H Detection Analysis Overview