Document Type
Article
Publication Date
2021
DOI
10.3389/fmed.2021.705071
Publication Title
Frontiers in Medicine
Volume
8
Pages
705071 (1-13)
Abstract
RNA sequencing (RNAseq) is a recent technology that profiles gene expression by measuring the relative frequency of the RNAseq reads. RNAseq read counts data is increasingly used in oncologic care and while radiology features (radiomics) have also been gaining utility in radiology practice such as disease diagnosis, monitoring, and treatment planning. However, contemporary literature lacks appropriate RNA-radiomics (henceforth, radiogenomics) joint modeling where RNAseq distribution is adaptive and also preserves the nature of RNAseq read counts data for glioma grading and prediction. The Negative Binomial (NB) distribution may be useful to model RNAseq read counts data that addresses potential shortcomings. In this study, we propose a novel radiogenomics-NB model for glioma grading and prediction. Our radiogenomics-NB model is developed based on differentially expressed RNAseq and selected radiomics/volumetric features which characterize tumor volume and sub-regions. The NB distribution is fitted to RNAseq counts data, and a log-linear regression model is assumed to link between the estimated NB mean and radiomics. Three radiogenomics-NB molecular mutation models (e.g., IDH mutation, 1p/19q codeletion, and ATRX mutation) are investigated. Additionally, we explore gender-specific effects on the radiogenomics-NB models. Finally, we compare the performance of the proposed three mutation prediction radiogenomics-NB models with different well-known methods in the literature: Negative Binomial Linear Discriminant Analysis (NBLDA), differentially expressed RNAseq with Random Forest (RF-genomics), radiomics and differentially expressed RNAseq with Random Forest (RF-radiogenomics), and Voom-based count transformation combined with the nearest shrinkage classifier (VoomNSC). Our analysis shows that the proposed radiogenomics-NB model significantly outperforms (ANOVA test, p < 0.05) for prediction of IDH and ATRX mutations and offers similar performance for prediction of 1p/19q codeletion, when compared to the competing models in the literature, respectively.
Original Publication Citation
Shboul, Z. A., Diawara, N., Vossough, A., Chen, J. Y., & Iftekharuddin, K. M. (2021). Joint modeling of RNAseq and radiomics data for glioma molecular characterization and prediction. Frontiers in Medicine, 8, 1-13, Article 705071. https://doi.org/10.3389/fmed.2021.705071
Repository Citation
Shboul, Zeina A.; Diawara, Norou; Vossough, Arastoo; Chen, James Y.; and Iftekharuddin, Khan M., "Joint Modeling of RNAseq and Radiomics Data for Glioma Molecular Characterization and Prediction" (2021). Electrical & Computer Engineering Faculty Publications. 291.
https://digitalcommons.odu.edu/ece_fac_pubs/291
ORCID
0000-0002-1277-4041 (Shboul), 0000-0002-8403-6793 (Diawara)
Included in
Biomedical Commons, Genetics Commons, Mathematics Commons, Radiology Commons
Comments
Copyright © 2021 Shboul, Diawara, Vossough, Chen and Iftekharuddin.
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