Document Type

Article

Publication Date

2024

DOI

10.1371/journal.pone.0287206

Publication Title

PloS ONE

Volume

19

Issue

1

Pages

e0287206 (1-17)

Abstract

We identified two different inherited mutations in KCNH2 gene, or human ether-a-go-go related gene (hERG), which are linked to Long QT Syndrome. The first mutation was in a 1-day-old infant, whereas the second was in a 14-year-old girl. The two KCNH2 mutations were transiently transfected into either human embryonic kidney (HEK) cells or human induced pluripotent stem-cell derived cardiomyocytes. We performed associated multiscale computer simulations to elucidate the arrhythmogenic potentials of the KCNH2 mutations. Genetic screening of the first and second index patients revealed a heterozygous missense mutation in KCNH2, resulting in an amino acid change (P632L) in the outer loop of the channel and substitution at position 428 from serine to proline (S428P), respectively. Heterologous expression of P632L and S428P into HEK cells produced no hERG current compared to the wild type (WT). Moreover, the co-transfection of WT and P632L yielded no hERG current; however, the co-transfection of WT and S428P yielded partial hERG current. Action potentials were prolonged in a complete or partial blockade of hERG current from computer simulations which was more severe in Purkinje than ventricular myocytes. Three dimensional simulations revealed a higher susceptibility to reentry in the presence of hERG current blockade. Our experimental findings suggest that both P632L and S428P mutations may impair the KCNH2 gene. The Purkinje cells exhibit a more severe phenotype than ventricular myocytes, and the hERG current blockade renders the ventricles an arrhythmogenic substrate from computer modeling.

Rights

© 2024 Owusu-Mensah et al.

This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability

Article states: "All relevant data are within the paper and its Supporting Information files."

Original Publication Citation

Owusu-Mensah, A., Treat, J., Bernardi, J., Pfeiffer, R., Goodrow, R., Tsevi, B., Lam, V., Audette, M., Cordeiro, J. M., & Deo, M. (2024). Identification and characterization of two novel KCNH2 mutations contributing to Long QT syndrome. PloS ONE, 19(1), 1-17, Article e0287206. https://doi.org/10.1371/journal.pone.0287206

ORCID

0000-0002-9770-3356 (Owusu-Mensah),

Additional Files
Audette-2024-IdentificationandCharacterizationSupplement.docx (1005 kB)
Supporting Information File- https://doi.org/10.1371/journal.pone.0287206.s001
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