Document Type
Article
Publication Date
2013
DOI
10.1371/journal.pone.0062019
Publication Title
PLoS ONE
Volume
8
Issue
4
Pages
e62019 (1-7)
Abstract
Experiments were conducted to redirect mouse Embryonic Stem (ES) cells from a tumorigenic phenotype to a normal mammary epithelial phenotype in vivo. Mixing LacZ-labeled ES cells with normal mouse mammary epithelial cells at ratios of 1:5 and 1:50 in phosphate buffered saline and immediately inoculating them into epithelium-divested mammary fat pads of immune-compromised mice accomplished this. Our results indicate that tumorigenesis occurs only when normal mammary ductal growth is not achieved in the inoculated fat pads. When normal mammary gland growth occurs, we find ES cells (LacZ+) progeny interspersed with normal mammary cell progeny in the mammary epithelial structures. We demonstrate that these progeny, marked by LacZ expression, differentiate into multiple epithelial subtypes including steroid receptor positive luminal cells and myoepithelial cells indicating that the ES cells are capable of epithelial multipotency in this context but do not form teratomas. In addition, in secondary transplants, ES cell progeny proliferate, contribute apparently normal mammary progeny, maintain their multipotency and do not produce teratomas.
ORCID
0000-0003-3329-9478 (Bruno)
Original Publication Citation
Boulanger, C.A., Bruno, R.D., Mack, D.L., Gonzales, M., Castro, N.P., Salomon, D.S., & Smith, G.H. (2013). Embryonic stem cells are redirected to non-tumorigenic epithelial cell fate by interaction with the mammary microenvironment. PLoS ONE, 8(4 ), e62019 doi: 10.1371/journal.pone.0062019
Repository Citation
Boulanger, Corinne A.; Bruno, Robert D.; Mack, David L.; Gonzales, Monica; Castro, Nadia P.; Salomon, David S.; and Smith, Gilbert H., "Embryonic Stem Cells Are Redirected to Non-Tumorigenic Epithelial Cell Fate by Interaction with the Mammary Microenvironment" (2013). School of Medical Diagnostics & Translational Sciences Faculty Publications. 16.
https://digitalcommons.odu.edu/medicaldiagnostics_fac_pubs/16
Included in
Animal Experimentation and Research Commons, Cancer Biology Commons, Cell Anatomy Commons, Developmental Biology Commons