Document Type

Article

Publication Date

2019

DOI

10.1038/s41598-019-43922-z

Publication Title

Scientific Reports

Volume

9

Pages

7466 (1-10)

Abstract

The normal mammary microenvironment can suppress tumorigenesis and redirect cancer cells to adopt a normal mammary epithelial cell fate in vivo. Understanding of this phenomenon offers great promise for novel treatment and detection strategies in cancer, but current model systems make mechanistic insights into the process difficult. We have recently described a low-cost bioprinting platform designed to be accessible for basic cell biology laboratories. Here we report the use of this system for the study of tumorigenesis and microenvironmental redirection of breast cancer cells. We show our bioprinter significantly increases tumoroid formation in 3D collagen gels and allows for precise generation of tumoroid arrays. We also demonstrate that we can mimic published in vivo findings by co-printing cancer cells along with normal mammary epithelial cells to generate chimeric organoids. These chimeric organoids contain cancer cells that take part in normal luminal formation. Furthermore, we show for the first time that cancer cells within chimeric structures have a significant increase in 5-hydroxymethylcytosine levels as compared to bioprinted tumoroids. These results demonstrate the capacity of our 3D bioprinting platform to study tumorigenesis and microenvironmental control of breast cancer and highlight a novel mechanistic insight into the process of microenvironmental control of cancer.

Comments

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

ORCID

0000-0003-2989-1292 (Reid, John), 0000-0002-1289-5302 (Palmer, Xavier-Lewis), 0000-0002-0621-2819 (Mollica, Peter), 0000-0002-0871-6789 (Sachs, Patrick), 0000-0003-3329-9478 (Bruno, Robert)

Original Publication Citation

Reid, J. A., Palmer, X. L., Mollica, P. A., Northam, N., Sachs, P. C., & Bruno, R. D. (2019). A 3D bioprinter platform for mechanistic analysis of tumoroids and chimeric mammary organoids. Scientific Reports, 9, 7466. doi:10.1038/s41598-019-43922-z

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