Document Type
Article
Publication Date
10-1989
Publication Title
Journal of Cell Science
Volume
94
Issue
2
Pages
361-369
Abstract
Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP antibodies. However, treatment of cultures with purified collagenase, unmasks immunoreactive sites and permits strong cell surface immunolabeling. This result suggests that collagen sterically blocks antibody access to CBP. Finally, we show that antibodies to CBP recognize purified avian integrin beta subunits; and that antibodies to avian integrins recognize a 100,000 Mr CBP. These data demonstrate that chicken embryonic fibroblasts possess surface polypeptides that mediate adhesion to type I collagen, and suggest that two of these proteins are related to the integrin family.
ORCID
0000-0002-4231-9488 (Ogle)
Original Publication Citation
Ogle, R.C., Potts, A.J., Yacoe, M., & Little, C.D. (1989). Embryonic chicken fibroblast collagen binding proteins: Distribution, role in substratum adhesion, and relationship to integrins. Journal of Cell Science, 94(2), 361-369.
Repository Citation
Ogle, Roy C.; Potts, A. Jeannette; Yacoe, Marchall; and Little, Charles D., "Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role in Substratum Adhesion, and Relationship to Integrins" (1989). School of Medical Diagnostics & Translational Sciences Faculty Publications. 4.
https://digitalcommons.odu.edu/medicaldiagnostics_fac_pubs/4
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