The Effect of Pulse Duration on Mitochondrial Stability and ER Stress
Description/Abstract/Artist Statement
Pulsed electric fields (PEF) as a treatment have shown promising results in a variety of applications including tumor ablation, electrochemotherapy, gene therapy, and many other medical applications. Delivering pulsed electric field shocks disrupt a cell’s membrane barrier, which is known as electroporation. The permeabilization of the membrane can allow for the delivery of drugs or DNA into cells. However, if electroporation causes damage that exceeds the cell's capacity to repair itself, several compounding factors including a loss in membrane potential lead to cell death. We are investigating whether pulsed electric fields activate type I interferons. Specifically, we hypothesize that shorter (nanosecond) electric pulses disrupt intracellular membrane-based organization and compartmentalization, resulting in mitochondrial DNA circulation and type I interferon activation. In our experiments, PEF are delivered to CT-26 tumor cells or macrophages in electroporation cuvettes. Both trains of 200 ns and 100 μs pulses are tested in order to measure how electric stress affects cell death and production of IFN-β.
Faculty Advisor/Mentor
Dr. Claudia Muratori
Faculty Advisor/Mentor Department
Electrical and Computer Engineering Department
College Affiliation
College of Engineering & Technology (Batten)
Presentation Type
Oral Presentation
Disciplines
Bioelectrical and Neuroengineering | Biomedical Engineering and Bioengineering | Electrical and Computer Engineering
Session Title
Colleges of Business, Education, Engineering Presentations
Location
Learning Commons @Perry Library, Room 1310
Start Date
3-25-2023 9:30 AM
End Date
3-25-2023 10:30 AM
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The Effect of Pulse Duration on Mitochondrial Stability and ER Stress
Learning Commons @Perry Library, Room 1310
Pulsed electric fields (PEF) as a treatment have shown promising results in a variety of applications including tumor ablation, electrochemotherapy, gene therapy, and many other medical applications. Delivering pulsed electric field shocks disrupt a cell’s membrane barrier, which is known as electroporation. The permeabilization of the membrane can allow for the delivery of drugs or DNA into cells. However, if electroporation causes damage that exceeds the cell's capacity to repair itself, several compounding factors including a loss in membrane potential lead to cell death. We are investigating whether pulsed electric fields activate type I interferons. Specifically, we hypothesize that shorter (nanosecond) electric pulses disrupt intracellular membrane-based organization and compartmentalization, resulting in mitochondrial DNA circulation and type I interferon activation. In our experiments, PEF are delivered to CT-26 tumor cells or macrophages in electroporation cuvettes. Both trains of 200 ns and 100 μs pulses are tested in order to measure how electric stress affects cell death and production of IFN-β.