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Aquatic salamanders represent an important component of Virginia river watersheds, but despite potential declines, few specifics are known about their habitat preferences. We surveyed the habitats of the northern two-lined salamander and collected data on an array of habitat variables associated with the species. We used a logistic regression analysis to develop a model predicting its presence or absence for a given 50m-transect. Our final model incorporated the variation in stream depth and direction of stream flow and accounted for 25% of the variation in our data. We conclude that stream depth variation is an important feature of salamander habitat ecology, and surmise that direction of flow is of site-specific importance possibly related to stream order. Both features may be behavioral adaptations to avoid fish predation.