Document Type
Article
DOI
10.25778/XJDJ-2646
Abstract
Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR–ATR) was used to analyze pure and adulterated honey samples. The FTIR spectra was analyzed using principal component analysis (PCA) and partial least squares (PLS) regression analysis to determine if these methods could differentiate between pure, commercial, and sucrose-adulterated honey samples. PCA showed a clear distinction between pure and adulterated honey samples. Commercial honey samples showed clustering around the unadulterated samples. PLS regression analysis correctly identified 81.8% of the standards and samples used in the PCA analysis. The five commercial samples were tested and shown to have a concentration of less than 3% adulterant, which is likely due to differences in sucrose concentration between batches from different locations and bee types. PCA and PLS methods provide a quick and easy analysis of honey samples.
Recommended Citation
Roberts, D. T. and Kittredge, K. (2022). Using FTIR-ATR and chemometric methods to detect sucrose adulteration in commercial honey samples. Virginia Journal of Science, 73(3-4), 8pp. https://doi.org/10.25778/XJDJ-2646