Date of Award

Fall 1983

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemical Sciences

Committee Director

Frank E. Scully, Jr.

Committee Member

William Cook

Committee Member

Patricia A. Pleban

Committee Member

Maxwell A. Bempong

Call Number for Print

Special Collections LD4331.C45H88

Abstract

N,N'-Diphenylguanidine (DPG) is a potentially toxic compound used in the vulcanization of rubber products. Distribution of DPG in hybrid mice (C57BL/6J interbred with DAB2) was determined following intraperitoneal injection. Urine, fecal material, whole blood and tissues were collected at various times after administration of DPG and the extracts analyzed for DPG by high pressure liquid chromatography. Recovery of DPG from spiked urine and whole blood exceed 90%, however, the recovery of DPG from spiked tissue samples was approximately 70%.

DPG concentration in whole blood reached a maximum within ten minutes and rapidly decreased through 180 minutes after administration of DPG. The maximum concentration of DPG was found during the first two hours and the first 24 hours after administration in urine and feces respectively. In tissues the maximum concentration of DPG was found as five minutes after administration at which time 41% of the administered dose was recovered. Throughout the collection periods there was a high concentration of DPG in the kidneys, while the distribution of DPG throughout the remaining tissues decreased. By 180 minutes after administration only 0.2% of the administered dose was recovered in tissues.

A metabolite of DPG (MDPG) was identified during the in vitro incubation of DPG with mouse liver S9 fraction. MDPG was isolated and characterized using nuclear magnetic resonance and infrared absorption spectra. These spectra suggest that MDPG is a mono-para-hydroxylated DPG molecule. The distribution of MDPG in urine and tissues was determined.

The present data demonstrate that following IP administration to mice DPG is rapidly absorbed, distributed through the body, and rapidly metabolized to MDPG.

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DOI

10.25777/8bwn-ff65

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