Date of Award

Spring 1988

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

Frank E. Scully, Jr.

Committee Member

Mark S. Elliot

Committee Member

Patricia A. Pleban

Call Number for Print

Special Collections LD4331.C45N52

Abstract

Gastric fluid was recovered from Sprague-Dawley rats that had been fasted for 24 hr or 48 hr and administered 4 mL deionized water. The protein, carbohydrate and chloride ion content of the gastric fluid was determined.

When gastric fluid is chlorinated with radiolabeled chlorine to concentrations between 323 and 375 mg/L, analyzed by high performance chromatography followed by liquid scintillation counting, as much as 59% of the radiolabeled chlorine was associated with organic components of the gastric fluid.

Model studies with the aromatic amino acids and the protein pepsin showed the formation of chlorinated analogs. Two chlorinated amino acid derivatives of tyrosine were detected by high performance chromatography. Further characterization of the chlorination products was accomplished by identification of 36Cl-labeled analogs.

When gastric fluid was mixed with solutions of aqueous chlorine of concentrati.one between 20 and 200 mg/L tyrosine residues present in proteinaceous components of the fluid were chlorinated. Two chlorinated amino acid derivatives of tyrosine were identified by co-chromatography with known standards. Further characterization of the products of the chlorination of gastric fluid were accomplished by identification of 36Cl labeled products.

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DOI

10.25777/jn72-df44

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